Bayer Professor of Engineering and Director of the Center for Microfluidics and Medical Diagnostics
University of Notre Dame
MICRO/NANOFLUIDICS FOR LIQUID BIOPSY AND THERAPY DISCOVERY
Professor Hsueh-Chia Chang's group works on micro/nanofluidics and molecular sensing technologies for cancer screening and therapy development. The technologies they currently work on include fractionation/purification of biomarker nanocarriers (exosomes, ribonucleoproteins and lipoproteins); nanoporous membrane hybridization and CRISPR molecular sensors, solid-state nanopores for precise quantification of microRNA biomarkers, a nanocone TIRF array for real-time multi-plexed microRNA/protein profiling, surface acoustic wave modules for reagent-free cell/vesicle lysing, non-optical real-time and isothermal replacement hybridization PCR, high-throughput electrokinetic droplet microfluidics for digital PCR and conformal cell encapsulation, photoconductive/nanowire transistor sensors for protein quantification, high-yield micro-filters for circulating tumor cells and manufacturing strategies to integrate these technologies into functioning biochips for dedicated instruments. The emphasis is on low-cost and portable devices that can be used by lay people, without significant loss in yield, sensitivity and selectivity. Current applications are for screening of pancreatic, liver, lung, ovarian and breast cancers and for discovery of tumor cell immune checkpoint proteins for immunotherapy.